Extended Data Fig. 4: MMP-7 in the conditioned media of senescent A549 cells was enzymatically active. | Nature Aging

Extended Data Fig. 4: MMP-7 in the conditioned media of senescent A549 cells was enzymatically active.

From: Urinary detection of therapy-induced senescence and fibrosis using an injectable albumin-based nanoprobe

Extended Data Fig. 4: MMP-7 in the conditioned media of senescent A549 cells was enzymatically active.The alternative text for this image may have been generated using AI.

a. Schematic illustration of the commercial MMP-7 activity assay used in this study. Created in BioRender. Fruk, L. (2026) https://BioRender.com/boen294. b. Secreted MMP-7 was active as enzyme, as measured by fluorescence-based assay (n = 3 independent biological replicates per group, mean ± s.d., two-way ANOVA with Dunnett’s multiple comparisons). c. Graph of fluorophore liberated due to MMP-7 activity (n = 3 independent biological replicates per group, mean ± s.d., one-way ANOVA with Dunnett’s multiple comparisons). d. Photograph of uncropped human protease inhibitor array panels of CM samples from untreated and treated A549 cells. All blots were exposed for 2 min. e. Pixel intensity z-score of secreted TIMP corresponding to (d) (n = 3 independent biological replicates per group, mean ± s.d., two-way ANOVA with Tukey’s multiple comparisons).

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