Extended Data Fig. 10: Peroxidase assay enables spectroscopic urinary detection of pulmonary fibrosis, and urinary signals from both alloy assay and peroxidase assay correlate with tissue and circulating MMP-7 levels.
Initial kinetic values (A652nm/s) from peroxidase assay of urine samples collected from untreated or bleomycin-treated mice 2 h p.i. with nanoprobes (n = 8 independent mice per group, mean ± s.e.m, unpaired two-tailed t-test). b. Photograph of the peroxidase assay on urine samples from untreated or bleomycin-treated mice injected with nanoprobes. c. ROC analysis showing the diagnostic specificity and sensitivity of nanoprobes and alloy formation assay in detecting fibrosis between bleomycin-treated and untreated mice groups (AUC = 0.89, 95% CI = 0.7215 – 1, n = 8 independent mice). The solid line represents the ROC curve, the dashed diagonal line represents the performance of a random classifier (AUC = 0.5). d. Correlation analysis between the percentage of Masson’s+ area and α-SMA+ cells in the lungs and intensity of signals of the urine samples detected from peroxidase assay. Solid lines indicate linear regression fits (least-squares fit; estimated mean relationship between variables), and dashed lines denote the 95% confidence intervals of the regression line; n = 8 independent mice, two-tailed Pearson’s correlation. e. Correlation analysis between the percentage of senescence marker, p21+ and p16+, cells with the urinary signal from peroxidase assay. Solid lines indicate linear regression fits (least-squares fit; estimated mean relationship between variables), and dashed lines denote the 95% confidence intervals of the regression line; n = 8 independent mice, two-tailed Pearson’s correlation. Pearson’s coefficient (r) and P-values: r = 0.4308, P = 0.2866 for p21; r = 0.8374, P = 0.0095 for p16. Correlation analysis between the percentage of MMP-7+ cells in the lungs (f, g) and MMP-7 in the serum (h, i) and intensity of signals of the urine samples detected from alloy formation assay (f, h) and peroxidase assay (g, i). Solid lines indicate linear regression fits (least-squares fit; estimated mean relationship between variables), and dashed lines denote the 95% confidence intervals of the regression line; n = 8 independent mice, two-tailed Pearson’s correlation for (f)-(i). r = Pearson’s coefficient.
