Extended Data Fig. 2: Senescent human and murine cancer cells abundantly secrete MMP-7 into the conditioned media, while fibroblast cells do not.
a. Photograph of representative uncropped human protease array panels of conditioned media samples from untreated and treated A549 cells, as well as only media without cells (control). All blots are exposed for 2 min. Experiments involved n = 4 independent biological replicates per group, corresponding to the plot in Fig. 1c. b. Photograph of representative uncropped human protease array panels of conditioned media samples from untreated and treated HPF-a cells. Both blots are exposed for 3 min. Experiments involved n = 3 independent biological replicates per group, corresponding to the plot in Extended Data Fig. 1c. c. Pixel intensity z-score of secreted proteases corresponding to (b) (n = 3 independent biological replicates per group, mean ± s.d., ordinary two-way ANOVA with Šídák’s multiple comparisons). d. Western blot analysis of pro-MMP-7 level (28 kDa) from lysates of senescent and non-senescent A549 cells (n = 3 independent biological replicates yielding similar results) e. Log2 fold change gene expression levels of MMP7 in treated A549 cells relative to untreated cells (n = 3 independent biological replicates per group, mean ± s.d.). Concentration of MMP-7 in the conditioned media of untreated (non-senescent) and treated (senescent) (f) L1475 cells (n = 3 independent biological replicates per group, mean ± s.d.; unpaired two-tailed t-test) and (g) HPF-a cells (n = 3 independent biological replicates per group, mean ± s.d., statistical analysis not performed because both groups demonstrated identically undetectable levels of MMP-7). pRb: hyperphosphorylated Retinoblastoma.
