Extended Data Fig. 4: In vitro non-enzymatic formation of 3-HPA-S-SidD (ACP) and methylmalonyl-S-SidD. | Nature Synthesis

Extended Data Fig. 4: In vitro non-enzymatic formation of 3-HPA-S-SidD (ACP) and methylmalonyl-S-SidD.

From: Analysis of siderochelin biosynthesis reveals that a type II polyketide synthase catalyses diketide formation

Extended Data Fig. 4

Reaction mixtures were subjected to HPLC-HR-MS analysis after Glu-C digestion to determine the ratio between acylated SidD and holo-SidD. (a) Examination of 3-HPA-S-P2 and P2. Reactions were conducted in the mixture that contains SidD and 3-HPA-CoA. (b) Examination of methylmalonyl-S-P2 and P2. Reactions were conducted in the mixture that contains SidD and methylmalonyl-CoA. ACP, acyl carrier protein; 3-HPA, 3-Hydroxypicolinic acid; P, peptide; CoA, coenzyme A; HPLC, high performance liquid chromatography; HR, high resolution; MS, mass spectrometry; and NL, normalized level.

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