Fig. 5: Synthesis and evaluation of an AMPAR-based fluorescent MMP9 sensor in HEK293T cells. | Nature Synthesis

Fig. 5: Synthesis and evaluation of an AMPAR-based fluorescent MMP9 sensor in HEK293T cells.

From: In-brain synthesis of receptor-based protease sensors by coupling ligand-directed chemistry and click chemistry

Fig. 5

a, Schematic illustration of the analysis of MMP9 activity proximal to AMPARs. The image of the AMPAR was rendered using Pymol with data from PDB: 5L1F. b, Chemical structure of fluorescent peptide probes for the detection of MMP9 and CatB activity. c,d, Time-course analysis of MMP9 activity using an AMPAR-based fluorescent MMP9 sensor. CLSM observations were conducted at different times after the addition of collagenase. Fluorescence imaging was performed using a Leica CLSM equipped with a 40× objective. Scale bar, 10 µm. e, Plot of Ax555/Ax647 values on the cell surface versus time. Region of interest (ROI) (n = 5). Data are presented as means ± s.e.m.

Source data

Back to article page