Fig. 7: Evaluation of an AMPAR-based fluorescent MMP9 sensor in a living mouse brain. | Nature Synthesis

Fig. 7: Evaluation of an AMPAR-based fluorescent MMP9 sensor in a living mouse brain.

From: In-brain synthesis of receptor-based protease sensors by coupling ligand-directed chemistry and click chemistry

Fig. 7

a, Hippocampus area, 5× objective. Scale bar, 0.5 mm. b, Hilus area of the hippocampal dentate gyrus, 63× objective (numerical aperture, 1.4). Scale bar, 5 μm. Similar images were observed in at least three independent experiments. c, Boxplot of the Ax555/Ax647 ratio values of the fluorescent bright spots in the hilus area of the hippocampal dentate gyrus. The number of ROIs was 240 for each of the images (n = 240). The horizontal line and × within each box indicate the median and mean, respectively. Boxes show interquartile range (IQR). Whiskers show 1.5 × IQR. d, Cerebellum area, 5× objective. Scale bar, 1 mm. e, Area near Purkinje cells, 63× objective. Scale bar, 5 μm. Similar images were observed in at least three independent experiments. f, Boxplot of the Ax555/Ax647 ratio values of the fluorescent bright spots in the posterior lobe IX of the cerebellum. The number of ROIs was 240 for each of the images (n = 240). The horizontal line and × within each box indicate the median and mean, respectively. Boxes show IQR. Whiskers show 1.5 × IQR.

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