Extended Data Fig. 7: Comparation of CatM-6b, CatM-W86A-6a and SacM-3 crystal structures.

a, Overlay of CatM-6b (lime) and CatM-W86A-6a (light blue). The two flexible loop regions are highlighted by red circles. b, the overlay of CatM-6b and CatM-W86A-6a shows a steric clash between W86 and ligand 6a. c, The crystal structure of CatM-W86A-6a. No water molecule was built within 4 Å of ligand 6a, by Phenix. d, The crystal structure of SacM-3. One water molecule was built in the active cavity by Phenix, which may function as general base for the deprotonation of key lysine. Similar water molecules were also observed in crystal structures of AkaM, SacM, CatM, MicM and SacM-6b.