Extended Data Fig. 8: TRIM21 mediates ubiquitination of MCM8 and the mitochondria pore proteins.
a, Immunofluorescence and statistical analysis showing distribution of TRIM21 and TOM20 under indicated conditions in HeLa cells (n = 6 independent images). For box plots, the box extends from the Q1 to Q3 quartile values of the data, with a line at the median. The whiskers extend from minimum to maximum value of data. b, Immunoblot showing nuclear or cytosolic MCM8 in ctrl- or TRIM21- siRNA transfected THP-1 cells under indicated conditions. c. Immunoprecipitation and immunoblot showing interaction of MCM8 with LC3 in WT or TRIM21−/− THP-1 cells under indicated conditions. d, e, Immunoblot showing ubiquitination of VDAC1 (d) or BAK I in ctrl- or TRIM21-siRNA transfected THP-1 cells under indicated conditions. f, g, Immunoblot showing ubiquitination of VDAC1 (f) or BAK (g) in ctrl- or MCM8-siRNA transfected THP-1 cells under indicated conditions. h, Immunoblot showing ubiquitination of MCM8 (FL) or MCM8 (LIR) in EV or Myc-TRIM21 transfected HeLa cellI. i, Structural remodelling showing Lysine 208 and 232 at the interface of the MCM8-MCM9 hexamer ring structure. j, Dot plot comparing relative abundance of DLOOP and TERT in the cytosol of MCM8-WT, K208R, K232R, or K263R transfected HeLa cells under indicated conditions (n = 3 independent samples). Data are mean ± s.d. P values were calculated by two-tailed Student’s t test.
