Extended Data Fig. 9: Nitric oxide induces MCM8 mediated mitophagy. | Nature Cardiovascular Research

Extended Data Fig. 9: Nitric oxide induces MCM8 mediated mitophagy.

From: MCM8-mediated mitophagy protects vascular health in response to nitric oxide signaling in a mouse model of Kawasaki disease

Extended Data Fig. 9: Nitric oxide induces MCM8 mediated mitophagy.

a, Bar plot showing nitic oxide concentrations in THP-1 cells with or without NO scavenger 1400W treatment under indicated conditions (n = 3 independent samples). b, Immunoprecipitation and immunoblot showing ubiquitination of Flag-MCM8 in EV or myc-TRIM21 transfected HeLa cells with or without 1400W treatment. c, Immunoblot showing nuclear and cytosolic MCM8 in THP-1 cells with or without 1400W treatment under indicated conditions. d, Immunofluorescence and statistical analysis showing MCM8 distribution in HeLa cells with or without 1400W treatment under indicated conditions (n = 7 independent images). For box plots, the box extends from the Q1 to Q3 quartile values of the data, with a line at the median. The whiskers extend from minimum to maximum value of data. e, Representative H&E sections of WT or Nos2−/− mice heart and coronary artery tissues under PBS or LCWE treatment (n = 6 animals). f, Bar plots showing inflammatory cytokine concentrations in the plasma of WT or Nos2−/− mice at day 5 under PBS (WT, n = 10 animals; Nos2−/−, n = 6 animals) or LCWE (WT, n = 7 animals; Nos2−/−, n = 7 animals) treatment. Data are mean ± s.d. P values were calculated by two-tailed Student’s t test for all experiments.

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