Extended Data Fig. 1: MCM8 deficiency promotes IFN-I signaling and cardio vasculopathy. | Nature Cardiovascular Research

Extended Data Fig. 1: MCM8 deficiency promotes IFN-I signaling and cardio vasculopathy.

From: MCM8-mediated mitophagy protects vascular health in response to nitric oxide signaling in a mouse model of Kawasaki disease

Extended Data Fig. 1: MCM8 deficiency promotes IFN-I signaling and cardio vasculopathy.

a, CRISPR/Cas9 strategy used to generate Mcm8−/− mouse line. b, Gel electrophoresis showing representative genotyping data for WT and Mcm8−/− mice. c, Bar plots showing expressions of Nppa and Nppb from heart tissues of WT (PBS, n = 12 animals; LCWE, n = 12 animals; LCWE+anti-IFNAR, n = 8 animals), Mcm8+/− (PBS, n = 10 animals; LCWE, n = 8 animals; LCWE+anti-IFNAR, n = 6 animals) or Mcm8−/− (PBS, n = 6 animals; LCWE, n = 8 animals; LCWE+IFNAR, n = 8 animals) mice under indicated conditions. d, Bar plots comparing infiltration of neutrophils and macrophages from heart tissues of WT (PBS, n = 4 animals; LCWE, n = 5 animals; LCWE+anti-IFNAR, n = 4 animals), Mcm8+/− (PBS, n = 4 animals; LCWE, n = 5 animals; LCWE+anti-IFNAR, n = 4 animals) or Mcm8−/− (PBS, n = 3 animals; LCWE, n = 5 animals; LCWE+anti-IFNAR, n = 4 animals) mice under indicated conditions. e, CRISPR/Cas9 strategy used to generate Mcm8f/f mouse line. f, Representative H&E sections of heart and coronary artery tissues from Mcm8f/f, Mcm8f/f Cdh5Cre or Mcm8f/f Lyz2Cre mice under PBS or LCWE-treatment (n = 5 animals). For violin plots, middle line indicates median. g, Bar plots comparing infiltration of neutrophils and macrophages from heart tissues of Mcm8f/f (PBS, n = 8 animals; LCWE, n = 10 animals), Mcm8f/fCdh5Cre (PBS, n = 5 animals; LCWE, n = 8 animals) or Mcm8f/f Lyz2Cre (PBS, n = 8 animals; LCWE, n = 10 animals) mice under indicated conditions. h, Bar plots showing plasma cytokine concentrations at day 5 from Mcm8f/f (PBS, n = 8 animals; LCWE, n = 10 animals), Mcm8f/f Cdh5Cre (PBS, n = 5 animals; LCWE, n = 8 animals) or Mcm8f/f Lyz2Cre (PBS, n = 8 animals; LCWE, n = 10 animals) mice under PBS or LCWE treatment. i, Representative staining for Ifna4 and CD31 on heart tissues from Mcm8f/f (PBS, n = 8 animals; LCWE, n = 10 animals), Mcm8f/fCdh5Cre (PBS, n = 5 animals; LCWE, n = 8 animals) or Mcm8f/fLyz2Cre (PBS, n = 8 animals; LCWE, n = 10 animals) mice under PBS or LCWE treated conditions. Data are mean ± s.d. For box plots, the box extends from the Q1 to Q3 quartile values of the data, with a line at the median. The whiskers extend from minimum to maximum value of data. P values were calculated by two-tailed Student’s t test for all experiments.

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