Extended Data Fig. 3: Apoptotic Caspases suppress IRF3-IFN activation in MCM8 deficient cells. | Nature Cardiovascular Research

Extended Data Fig. 3: Apoptotic Caspases suppress IRF3-IFN activation in MCM8 deficient cells.

From: MCM8-mediated mitophagy protects vascular health in response to nitric oxide signaling in a mouse model of Kawasaki disease

Extended Data Fig. 3: Apoptotic Caspases suppress IRF3-IFN activation in MCM8 deficient cells.

a, Flow cytometry showing annexin V and PI staining of ctrl- or MCM8-siRNA transfected THP-1 cells. b, Immunoblot showing Caspase-3 activation in ctrl- or MCM8-siRNA transfected THP-1 cells under indicated conditions. c, d, ELISA showing IFN-β concentrations (n = 3 independent samples) (c) and immunoblot showing IRF3 phosphorylation (d) from ctrl- or MCM8-siRNA transfected THP-1 cells under indicated conditions. e, Flow cytometry (top) and quantitative analysis (bottom) comparing CCCP-depolarized mitochondria in ctrl- or MCM8-siRNA transfected THP-1 cells (n = 3 independent samples). Data are mean ± s.d. P values were calculated by two-tailed Student’s t test for all experiments.

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