Extended Data Fig. 3: Tbx5 overexpression atrializes ventricular myocytes. | Nature Cardiovascular Research

Extended Data Fig. 3: Tbx5 overexpression atrializes ventricular myocytes.

From: Tbx5 maintains atrial identity in postnatal cardiomyocytes by regulating an atrial-specific enhancer network

Extended Data Fig. 3

a, Strategy to generate TBX5-OE ventricles. A full length Tbx5 cDNA downstream of the ubiquitous CAG promoter was activated by the cardiomyocyte specific Myh6-Cre transgene. b-c, Volcano plot comparing the change in gene expression of mouse left ventricle overexpressing Tbx5 compared to control LV. aCM genes are marked in red in (a) and vCM genes are denoted in blue in (b). b-c, Wald’s test followed by Benjamini Hochberg correction. d-e, Fisher’s exact test (two tailed) was performed to determine if changes in chamber selective gene expression downstream of Tbx5 overexpression were significant. f-h, Staining for MYL4, MYL7, and MYL2 in atria and ventricles of control and TBX5-OE hearts. Staining for each marker was performed using 3 control hearts and 2 TBX5-OE hearts, and representative images are shown.

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