Extended Data Fig. 7: Characterization of myocyte differentially accessible regions.

Heatmap (a) shows the patterns of differential accessibility between myocyte clusters. The rows contain the union of regions with differential accessibility in the four pairwise comparisons shown in Fig. 5a. ATAC signal in each region is shown for myocyte clusters 1 (control_aCM cluster), 4, 6 (KO_aCM cluster), and 5. The regions are grouped (groups a-f) by their pattern of accessibility change in the four pairwise comparisons. Please also refer to Supp. Table 4. Arrows denote significant enrichment in one cluster compared to another. These six groups fit two predominant patterns: those with and those without predominant accessibility in the control aCM cluster (Myocyte_1). Most regions with predominant accessibility in the control aCM cluster were occupied by TBX5 and had GO terms related to cardiac cell development or striated muscle contraction (b). In contrast, a minority of regions without predominant accessibility in the control aCM cluster were occupied by TBX5 and had GO terms that were atypical for cardiomyocytes (c). Groups with less than 200 regions are not shown in the heatmap.