Extended Data Fig. 8: Proximity and predicted gene linkages demonstrate the regulation of the atrial GRN by control peaks. | Nature Cardiovascular Research

Extended Data Fig. 8: Proximity and predicted gene linkages demonstrate the regulation of the atrial GRN by control peaks.

From: Tbx5 maintains atrial identity in postnatal cardiomyocytes by regulating an atrial-specific enhancer network

Extended Data Fig. 8

a, The different types of association of a genomic region with a gene: (1) Linkage (L). Region-to-gene linkages are predicted based on co-variance of accessibility and expression on a nucleus-by-nucleus basis in the multiome data. (2) Proximity (P). Region-to-gene relationships are inferred by proximity of the region to the gene’s transcriptional start site. (3) Proximity and linkage (P + L). A region-to-gene association can be supported by both proximity and linkage. b, Genes were associated with control and KO regions by linkage (n = 2 control and 2 KO multiome biological replicates). Ratio of gene expression between the control and KO aCM clusters was plotted and compared between groups by the two-sided Mann-Whitney test. P = 9.1E-48. c, Association of control and KO ATAC regions with aCM and vCM genes. Associations were made based on proximity, linkage, or both. d, aCM and vCM genes were interrogated for associations with control and KO ATAC regions by proximity, linkage, or both.

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