Extended Data Fig. 2: Female Mice Treated with Doxorubicin Exhibit Cardiotoxicity and Cardiac CD8+ T-cell Infiltration.
From: Cytotoxic T cells drive doxorubicin-induced cardiac fibrosis and systolic dysfunction
A. Schematic of DR treatment in female mice (n = 7 mice / group). B-C. Echocardiography was used to measure ejection fraction and wall thickness one day prior to tissue collection. D. Whole heart weight normalized to tibia length. E-F. Formalin fixed cardiac samples were stained with TUNEL, representative images shown in E with scale bars of 50 μm quantified using ImageJ in F. G-J. Hearts were enzymatically and mechanically digested and analyzed for cardiac T-cells by flow cytometry (gating strategy in Supplementary Fig. 1). Quantification by frequency shown in G, I and by total cell number in H, J. K-L. Whole blood was analyzed by flow cytometry for circulating T-cells (gating strategy in Supplementary Fig. 1) quantified in K-L. All data shown are mean±SEM. Statistical analysis by 2 sided T-tests, exact p-values shown.
