Extended Data Fig. 5: Doxorubicin Increases Multiple Helper T-cell Subtypes in Lymphoid Organs.
From: Cytotoxic T cells drive doxorubicin-induced cardiac fibrosis and systolic dysfunction
A. Schematic showing helper T-cell differentiation and transcription factors probed to quantify each subtype (n = 7–9 mice / group). B. Gating strategy used to identify T-cell transcription factors in digested mediastinal lymph nodes (mLN) or spleens form PBS or DR-treated mice, with quantification of mLN Th17 cells (C), mLN Th2 cells (D), splenic Th17 cells (E), or splenic Th2 cells (F). G. Representative plots of splenic CD4+ Tbx21 staining from 8 week treated mice (gated on CD45+CD11b-CD4+ cells), with quantification for splenic Th1 cells (H) or mLN Th1 cells (I). All data shown are mean±SEM. Statistical analysis by 2-way ANOVA with Sidak’s multiple comparison test, exact p-values shown.
