Extended Data Fig. 1: Doxorubicin Effects on Cardiac and Circulating Myeloid Populations.
From: Cytotoxic T cells drive doxorubicin-induced cardiac fibrosis and systolic dysfunction
WT mice were treated with PBS or 5.0 mg/kg DR for 4 or 8 weeks (n = 7–10 mice / group). A. Hearts were digested and analyzed by flow cytometry, shown is the gating strategy used for T-cells and myeloid populations, and quantification by frequency or total cell number for CD11b+ cells (B,F), CD11b+CCR2+ cells (C,G), CD11b+Ly6G- cells (D,H), or CD11b+MertK+ cells (E, I). J. Whole blood was analyzed by flow cytometry, shown is gating strategy for T-cells and myeloid populations, and quantification for CD11b+CCR2+ cells (K), or CD11b+Ly6G- cells (L). All data shown are mean±SEM. Statistical analysis by 2-way ANOVA with Sidak’s multiple comparison test, exact p-values shown.
