Extended Data Fig. 2: RNA-seq and metabolomics analysis of M1cKO mouse hearts.
a. KEGG pathway enrichment analysis using the differentially expressed genes (DEGs) in 4-week-old M1cKO mouse hearts. b-c. Principal component analysis (PCA) of metabolites in negative (b) and positive (c) ion modes in 4-week-old control and M1cKO mouse hearts. d-e. Volcano plot of differential metabolites detected in negative (d) and positive (e) ion modes (including unidentified ones) in 4-week-old control and M1cKO mouse hearts. f. Grouping of metabolic hits identified by LC-MS/MS analysis in 4-week-old control and M1cKO mouse hearts (n = 5 hearts for each group). g. Quantification of citrate by LC-MS/MS analysis in control and M1cKO mouse hearts (n = 4 hearts for each group). h. Quantification of medium-chain fatty acids (MCFAs) and long-chain fatty acids (LCFAs) by LC-MS/MS analysis in control and M1cKO mouse hearts (n = 4 hearts for each group). i-j. Quantification of cholesterol (i) and TG (52:3) (j) by LC-MS/MS analysis in control and M1cKO mouse hearts (n = 4 hearts for each group). k. Quantification of C20:3 (M + 16) in control and M1KD NRVMs after [U-13C16] palmitate labeling for 24 h, as revealed by metabolic flux assay (n = 3 hearts for each group). Statistical analysis was evaluated using unpaired two-tailed Student’s t-test. The values are mean ± s.d. NS, not significant.
