Extended Data Fig. 5: Mettl1 regulates tRNA m7G modification but has minimal impact on other methylation reactions. | Nature Cardiovascular Research

Extended Data Fig. 5: Mettl1 regulates tRNA m7G modification but has minimal impact on other methylation reactions.

From: The tRNA methyltransferase Mettl1 governs ketogenesis through translational regulation and drives metabolic reprogramming in cardiomyocyte maturation

Extended Data Fig. 5: Mettl1 regulates tRNA m7G modification but has minimal impact on other methylation reactions.

a. Quantification of SAM metabolism-related metabolites by LC-MS/MS analysis in control and M1cKO mouse hearts (n = 4 hearts for each group). b. Dot blotting and quantification of N6-methyladenosine (m6A) and m7G levels in total RNA from control and M1cKO mouse hearts (n = 3 hearts for each group). c. Western blotting of protein with mono-methylation (left panels) (n = 2 hearts for each group, observed in 3 independent experiments), di-methylation (middle panels) (n = 3 hearts for each group, observed in 3 independent experiments) and tri-methylation (right panels) (n = 3 hearts for each group, observed in 3 independent experiments) of lysine residues in control and M1cKO mouse hearts. d. Western blotting of protein with symmetrical (left panels) and asymmetric (right panels) di-methylation of arginine residues in control and M1cKO mouse hearts (n = 3 hearts for each group, observed in 3 independent experiments). Statistical analysis was evaluated using unpaired two-tailed Student’s t-test. The values are mean ± s.d. NS, not significant. Uncropped blots for c-d are provided in Source data.

Source data

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