Extended Data Fig. 6: Mettl1 does not mediate translational regulation of FAO genes.
a. Western blotting and quantification of fatty acid oxidation proteins in 4-week-old control and M1cKO mouse hearts. b. mRNA expression of fatty acid oxidation genes measured by qRT-PCR in 4-week-old control and M1cKO mouse hearts. c. TE analysis of fatty acid oxidation genes based on qRT-PCR and western blotting in 4-week-old control and M1cKO mouse hearts. d. Western blotting and quantification of fatty acid oxidation proteins in control and M1cKO mouse hearts at P7. e. mRNA expression of fatty acid oxidation genes measured by qRT-PCR in control and M1cKO mouse hearts at P7. f. TE analysis of fatty acid oxidation genes based on qRT-PCR and western blotting in control and M1cKO mouse hearts at P7. n = 3 hearts for each group in (a-f). g. GSEA plot of oxidative phosphorylation pathway in RNA-Seq data upon control and M1cKO mouse hearts at P7. h. mRNA expression of oxidative phosphorylation genes measured by qRT-PCR in control and M1cKO mouse hearts at P7 (n = 4 hearts for each group). i. Western blotting and quantification of Uqcrc2 in control and M1cKO mouse hearts at P7 (n = 3 hearts for each group). Statistical analysis was evaluated using unpaired two-tailed Student’s t-test. The values are mean ± s.d. NS, not significant. Uncropped blots for a, d and i are provided in Source data.
