Extended Data Fig. 7: Mettl1 regulates the translation of Hmgcs2 but not Hmgcs2 enzyme activity and ketone oxidation.
a. Relative MS intensity of indicated proteins in P1, P4, P9, P23 mouse hearts (nP1 = 14 hearts, n4 = 14 hearts, nP9 = 10 hearts, nP23 = 10 hearts, missing values are eliminated) (GSE119530). b. Relative total enzyme activity of Hmgcs2 in control and M1cKO hearts (n = 3 hearts for each group). c. Western blotting and quantification of Hmgcs2 in control and M1cKO mouse hearts (n = 3 hearts for each group). d. Relative enzyme activity of Hmgcs2 normalized to the corresponding protein levels in control and M1cKO hearts (n = 3 hearts for each group). e-f. Western blotting and quantification of enzymes involved in ketone oxidation in control and M1cKO mouse hearts (e) (n = 3 hearts for each group) and in control and M1KD NRVMs (f) (n = 3 biological replicates for each group). g. Fraction of labeled acetylcarnitine from [U-13C]β-HB in control and M1cKO mouse hearts, as revealed by metabolic flux assay (n = 3 hearts for each group). Statistical analysis was evaluated using unpaired two-tailed Student’s t-test. The values are mean ± s.d. NS, not significant. Uncropped blots for c, e and f are provided in Source data.
