Extended Data Fig. 9: β-HB treatment promotes K-bhb modifications and rescues decreased activities of TCA cycle-related enzymes in M1-null H9C2 CMs. | Nature Cardiovascular Research

Extended Data Fig. 9: β-HB treatment promotes K-bhb modifications and rescues decreased activities of TCA cycle-related enzymes in M1-null H9C2 CMs.

From: The tRNA methyltransferase Mettl1 governs ketogenesis through translational regulation and drives metabolic reprogramming in cardiomyocyte maturation

Extended Data Fig. 9: β-HB treatment promotes K-bhb modifications and rescues decreased activities of TCA cycle-related enzymes in M1-null H9C2 CMs.

a-c. Relative enzyme activities of mitochondrial IDH (IDHm) (a), succinyl-CoA synthetase (SCS) (b) and cytoplasmic MDH (c) in OAPA-treated WT and M1-null H9C2 cardiomyocytes (n = 3 biological replicates for each group). d-g. Immunoprecipitation assays (d) and quantification (e-g) of PBS or β-HB-treated Mettl1-null H9C2 cardiomyocytes. IP: anti-K-hbb; IB: Idh2, Sucla2 and Mdh1. n = 3 biological replicates for each group. h-j. Relative enzyme activities of mitochondrial IDH (IDHm) (h), succinyl-CoA synthetase (SCS) (i) and cytoplasmic MDH (j) in PBS or β-HB-treated Mettl1-null H9C2 cardiomyocytes (n = 3 biological replicates for each group). Statistical analysis was evaluated using unpaired two-tailed Student’s t-test. The values are mean ± s.d. Uncropped blots for d are provided in Source data.

Source data

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