Extended Data Fig. 4: Allograft inflammatory factor 1 (AIF1) promotes Hypoxia Inducible Factor (HIF)-1α-dependent glycolysis after myocardial infarction (MI).
A HIF-1α activation in cardiac macrophages 3 days after MI (n = 3 day 0 MI and n = 6 day 3 MI, two-way ANOVA with Tukey’s test, pooled from two independent experiments). B Isolation of cardiac macrophages 3 days after MI with representative enrichment assessed by flow cytometry. C Extracellular acidification rate (ECAR) and oxygen consumption rate (OCR) by cardiac macrophages isolated from the infarct 3 days after MI (n = 4 for all groups, two-way ANOVA with Tukey’s test, two independent experiments). D Publicly available microarray data (GSE112630) of cytokine and glycolytic gene expression among CCR2+ and CCR2− macrophages isolated from the hearts of patients during ischemic (ICM) or dilated cardiomyopathy (DCM). Data are presented as mean ± SEM.
