Extended Data Fig. 5: Calcium influx is required for inflammatory glycolytic reprogramming of macrophages after TLR4 stimulation. | Nature Cardiovascular Research

Extended Data Fig. 5: Calcium influx is required for inflammatory glycolytic reprogramming of macrophages after TLR4 stimulation.

From: Mechanical regulation of macrophage metabolism by allograft inflammatory factor 1 leads to adverse remodeling after cardiac injury

Extended Data Fig. 5

A Calcium influx in bone marrow-derived macrophages (BMDM) after TLR4 or ionomycin (IM) stimulation. Images are representative of two independent experiments. Scale bar, 20 μm. B Cytokine production by BMDMs treated with a cell-permeable, calcium chelator (BAPTA) (n = 4 for IL-6 and TNF-α and n = 3 for IL-10, two-way ANOVA with Tukey’s test, three independent experiments). Extracellular acidification rate (ECAR) and oxygen consumption rate (OCR) in BMDMs after treatment with calcium chelators, C BAPTA or D EGTA. Vehicle treated groups are the same in C and D (n = 6 vehicle and n = 8 for all other groups, two-way ANOVA with Tukey’s test, two independent experiments). E ECAR and OCR in BMDMs treated with ionomycin (n = 5 for all groups, two-way ANOVA with Tukey’s test, two independent experiments). Data are presented as mean ± SEM.

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