Extended Data Fig. 6: The effects of NF110 overexpression on cardiomyocyte proliferation and the influence among NF45, NF110, and PA2G4.
a, NF110 overexpression promotes cardiomyocyte proliferation (n=3 independent experiment). b, The effects of PA2G4 siRNA on the expression of NF90 and TCP80 (n=8 independent experiment). c, The effects of PA2G4 overexpression on the expression of NF110 in cardiomyocytes. This experiment was repeated three times independently. d, The quantification of Western blot results indicating the effects of PA2G4 on the expression of NF110 and NF45 in the nucleus and cytoplasm in cardiomyocytes (n≥4 independent experiment). e and f, The effects of NF110 and NF45 siRNAs on the protein expression level of PA2G4, NF110, and NF45 in cardiomyocytes (n≥5 independent experiment). g, The inhibitory effect of PA2G4 overexpression on the expression of NF110 was blocked by MG132 in cardiomyocytes. This experiment was repeated three times independently. h, Secondary mass spectrum for RNF13. Data are presented as mean ± s.e.m. Statistical analyses were performed by Two-tailed Student’s t test with (b, d, e) or without Welch’s correction (a, b, d-f). NC siRNA, negative control for siRNA; OE, overexpression.
