Extended Data Fig. 7: The effects of CRAPIR on the expression of NF110 and NF45 in cardiomyocytes and knockdown of NF45 attenuates the effects of CRAPIR on cardiomyocyte proliferation.
a, The protein expression level of NF110 and NF45 in cardiomyocytes transfected with CRAPIR (n≥6 independent experiment). b, The effects of CRAPIR on the expression of NF110 and NF45 in the nucleus and cytoplasm analyzed by Western blot. This experiment was repeated three times independently. c, The proliferative ability of cardiomyocytes was analyzed by the detection of EdU, pH3 and Ki67 (n=3 independent experiment). d, Co-IP analysis revealing the ubiquitination of NF110 was induced by CRAPIR antagomir in cardiomyocytes. This experiment was repeated three times independently. Data are presented as mean ± s.e.m. Statistical analyses were performed by Two-tailed Student’s t test with or without Welch’s correction (a), one-way ANOVA followed by Tukey’s Multiple Comparison tests (c). NC siRNA, negative control for siRNA; ago, CRAPIR agomir; ago-NC, negative control for agomir; anta, CRAPIR antagomir; anta-NC, negative control for antagomir.
