Extended Data Fig. 4: IL-1β injection does not produces maturation of IL-1β in left atria of Casp1-/-.
a, Serum levels of IL-6 in saline- and IL-1β–injected Casp1–/– mice. b, Western blot of IL-1β/pro-IL-1β in Casp1-/- mice. Quantitation levels were normalized to glyceraldehyde 3-phosphate dehydrogenase (GAPDH) loading control. Each sample represents one mouse heart; 5 saline-injected and 6 IL-1β-injected, samples were analyzed. All the central lines in the box plots represent the median, the box limits represent the first and third quartiles and the whiskers denote the minimum and maximum values. P values were calculated with Mann-Whitney’s test (two-tailed) (a) and Student’s t-test (two-tailed) (b).
