Extended Data Fig. 7: IL-1β injection increases arrhythmia susceptibility in a mechanism dependent of Casp1 expression and not IL-6.
a, graphical scheme of experiments. Casp1-/- mice were treated for 15 days with saline or IL-1β subcutaneously (SC). b, representative EKG traces from saline and IL-1β treated mice. c, EKG parameter analyzed: RR interval, p wave duration, and PR interval. d, representative action potential traces from saline and IL-1β treated Casp1-/- mice. e, action potential duration (APD) at 30%, 50%, 70% and 90% of repolarization, from saline and IL-1β treated mice. Lower inset panel show adjusted mean ± 95% C.I.f, triggered activities in Casp1-/- mice. g, graphical scheme of IL-6 restitution in Casp1-/- mice injected with IL-1β. h, representative EKG trace in sinus rhythm after burst-pacing in Casp1-/- mice injected with IL-1β and IL-6. i, AF inducibility after burst-pacing in Casp1-/- mice injected with IL-1β and IL-6. All the central lines in the box plots represent the median, the box limits represent the first and third quartiles and the whiskers denote the minimum and maximum values. Categorical data are presented as bar plots (f,i). P values were calculated with Student’s t-test (two-tailed) (c), hierarchical analysis with mixed effect model (e), and Fisher’s exact test (f,i) (two-tailed).
