Extended Data Fig. 9: IL-1β injection does not produces maturation of IL-1β in left atria of Csf1rCre IL-1rfl/fl.

a, graphical scheme of experiments. Csf1rCre IL-1rfl/fl mice were treated for 15 days with saline or IL-1β subcutaneously (SC). b, Western blot of IL-1β/pro-IL-1β in Csf1rCre IL-1rfl/fl mice. Quantitation levels were normalized to glyceraldehyde 3-phosphate dehydrogenase (GAPDH) loading control. Each sample represents one mouse heart; 5 saline-injected and 6 IL-1β-injected samples were analyzed. All the central lines in the box plots represent the median, the box limits represent the first and third quartiles and the whiskers denote the minimum and maximum values. P values were calculated with Student’s t-test.