Extended Data Fig. 1: Global view of cardiac fibroblast single-cell RNA sequencing from human myocardial infarction heart.
a, UMAP dimensionality reduction plots of all 6989 sequenced cardiac fibroblasts from different cardiac areas of a MI patient. b, Enrichment of each fibroblast subset in human heart from infarct zone or remote area. c, Heatmap of top marker genes for each fibroblast subtype in UMAP. d, Venn diagram analysis of mouse cardiac F9 subset and human F5 subset. e, Quantification score of top 100 differentially expressed genes in mouse heart CD248+ fibroblast subset across different human heart fibroblast subsets. f, Immunofluorescence analysis of CD248 (red) expression in vimentin-marked cardiac fibroblasts from normal and infarcted human heart. g, Western-blot analysis of CD248 expression (by the Abcam antibody, no. 67273) in I/R-injured mouse hearts at different timepoints (sham, 3 days, 7 days, 14 days and 28 days post I/R). h, Different cell types were FACS-sorted from infarcted heart to analyze CD248 expression (by the Abcam antibody, no. 67273) by western-blot at 28 days post-infarction. i, Co-immunofluorescence staining of CD248 (red) with cardiomyocytes (Troponin I, green), endothelial cells (CD31, green), fibroblasts (Vimentin, green), T cells (CD3, green) and macrophage (CD68, green) in mouse heart 28 days post-infarction respectively. Scale bar = 20 μm.
