Extended Data Fig. 9: Differential gene expression in endothelial cells across time. | Nature Cardiovascular Research

Extended Data Fig. 9: Differential gene expression in endothelial cells across time.

From: Mapping the transcriptional and epigenetic landscape of organotypic endothelial diversity in the developing and adult mouse

Extended Data Fig. 9

a) UMAP of only endothelial cells (from Fig. 6) colored coded by timepoint and clustered using Monocle 3 to show maturation across time. b) Feature plots denoting normalized expression of endothelial marker genes, Pecam1 and Cdh5, and canonical lymphatic marker genes, Lyve1 and Prox1, demonstrate the preferential enrichment of blood endothelial cell transcriptomes rather than lymphatic endothelial transcriptomes. c) Heatmap of differential gene expression analysis of single cell RNA-seq results from brain endothelial cells profiled across developmental time (row z-score). Genes in red have a known role in blood brain barrier function. The top 10 differentially expressed genes for each time point are shown, followed by the pan-endothelial transcripts Pecam1, Cldn5 and Kdr (normalized expression). d) Expression of BBB-related genes over developmental time.

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