Extended Data Fig. 7: T-5224 blocks 27-HC-stimulated inflammatory memory in MH HUVECs.
a, Relative mRNA levels of TNF, NLRP3, and CCL3 in P1 and P6 HUVECs from control pregnant patients (Ctrl; pink) and those with MH (MH; blue). n = 6. b, CUT&RUN-qPCR analyses showing the interaction of JUN with the gene bodies of 3 genes in Ctrl and MH HUVECs. n = 6. c, d, In the presence of 27-HC, CUT&RUN-qPCR analysis showing the enrichment of JUN (c) and H3K27ac (d) at TNF, NLRP3, and CCL3 in Ctrl and MH HUVECs treated with siNC or siERα. n = 5. e, Relative TNF (left) and CCL3 (right) levels by ELISA assay in Ctrl and MH HUVECs treated with DMSO or T-5224 with or without 27-HC treatment. n = 6. f, Representative western blot (above) and quantification of NLRP3 protein levels (below) in Ctrl and MH HUVECs treated with DMSO or T-5224 with or without 27-HC treatment, normalized to GAPDH. n = 6. g, Relative TNF, NLRP3, and CCL3 mRNA levels in Ctrl and MH HUVECs treated with DMSO or T-5224 with or without ox-LDL treatment. n = 6. h, CUT&RUN-qPCR analysis showing the enrichment of JUN at TNF, NLRP3, and CCL3 in Ctrl and MH HUVECs treated with DMSO or T-5224 with or without ox-LDL treatment. n = 6. i, Ctrl and MH HUVECs were subjected to 27-HC treatment for 24 hours. The lysate was immunoprecipitated with p300, followed by western blots with the indicated antibodies. n = 3. Data are presented as the mean ± SEM. Ordinary one-way ANOVA with Sidak’s multiple comparison test (a-d) and ordinary two-way ANOVA with Sidak’s multiple comparison test (e-h) were used to determine statistical significance.
