Extended Data Fig. 2: Representative cardiac immune cell flow analysis gating strategies.
From: The immune checkpoint regulator CD40 potentiates myocardial inflammation
a, Gating strategy for macrophages starting with gating for immune cells (CD45+), followed by doublet exclusion, then live/dead exclusion, then macrophages (CD11b+, CD64+), then CCR2 macrophages (MHChiCCR2-GFP+). b, Gating strategy for T-cells starting with gating for immune cells (CD45+), followed by doublet exclusion, then T-cells (CD4+ or CD8+), then effector memory phenotype (CD4+CD44+ or CD8+CD44+). Gating strategies for c, dendritic cells (Zbtb46-GFP+), d, neutrophils (Ly6G+), and e, B cells (CD19+) are also shown.
