Extended Data Fig. 5: Low levels of anti-drug antibodies with minimal impact on the inflammatory response from serial injections.
From: The immune checkpoint regulator CD40 potentiates myocardial inflammation
a, Quantification of mouse anti-rat IgG levels from serum in naïve mice (n = 3), mice treated with 100 μg Rat IgG (n = 4) or 100 μg α-CD40 (n = 4) for 7 days, or mice treated with 20 μg α-CCR2 (n = 3) for 4 days. b, Experimental model of mice receiving 1 single or 3 serial anti-CD40 agonist antibody injections (Created in BioRender. Lavine, K. (2025) https://BioRender.com/e68h639). c, Ifng, and d, Cxcl9 mRNA expression measured by RT-qPCR from cardiac tissue in Rat IgG (n = 3), 1 injection of α-CD40 (n = 5), and 3 injections of α-CD40 (n = 4). One-way ANOVA with Sidak correction. Error bars indicate means ± s.e.m.
