Extended Data Fig. 5: Angiogenic transdifferentiation in vivo.
From: O-GlcNAcylation promotes angiogenic transdifferentiation to reverse vascular ischemia
a. Merged immunofluorescence images show the co-localization of YFP (green) with CD31 (red) in ischemic hindlimb tissue of Fsp1-Cre: R26R-EYFP mice at day 7 post-surgery. Images were captured at 40x (21 z-stacks, 0.5 µm/slice) and 100x magnification (17 slices, 0.5 µm/slice). Scale bar = 50 µm. b. Single-channel immunofluorescence images of DAPI (blue), YFP (green), and CD31 (red) in ischemic hindlimb tissue at day 7 post-surgery, captured at 40x magnification. c. Immunofluorescence images illustrate YFP + CD144+ (double-positive) cells in hindlimb muscle tissue of Fsp1-Cre: R26R-EYFP mice at day 7 post-surgery. Images were captured at 40x (15 z-stacks, 0.45 µm/slice) and 100x magnification (15 slices, 0.39 µm/slice). Scale bar = 50 µm. d. Single-channel immunofluorescence images of DAPI (blue), YFP (green), and CD144 (red) in ischemic hindlimb tissue at day 7 post-surgery, captured at 40x magnification. In a–d, results represent two independent experiments.
