Extended Data Fig. 9: Angiogenic transdifferentiation in vivo.
From: O-GlcNAcylation promotes angiogenic transdifferentiation to reverse vascular ischemia
a. Negative control for secondary antibody staining performed without primary antibody (Blue: DAPI, Red: lectin). b. Whole mount staining images show YFP + CD31+ double-positive cells incorporated into lectin-perfused vessels in the hindlimb muscle tissue at day 7 post-surgery. (Red: lectin; Green: YFP; Gray: CD31; Blue: DAPI; Scale bar = 50 µm, 14 z-stacks, [1 µm/slice]). c. Single-channel and merged immunofluorescence images of whole mount staining of DAPI, YFP, CD31, and lectin in ischemic hindlimb at day 7 post-surgery, captured at 20x magnification. d. Representative flow cytometry plots show a reduction in YFP + CD31+ cell population in the ischemic limb of OSMI-4-treated groups compared to vehicle-treated groups at day 7 post-surgery (n = 3 mice per group, results are representative of two independent experiments). In a-c, results represent two independent experiments.
