Extended Data Fig. 7: Single-nucleus RNA-seq analysis of patients with HF.
Related to Fig. 6. a: t-distributed stochastic neighbour embedding (tSNE) plot of human heart single-cell transcriptomes. The cells (dots) are coloured according to the cell clusters, and the numbers represent cluster numbers. b: Classification of whole heart cells based on gene expression using tSNE dimensionality reduction, identifying marker genes for cardiomyocytes, cardiac fibroblasts, endothelial cells, smooth muscle cells, myeloid cells, and lymphoid cells. c: tSNE plot of human heart single-cell transcriptomes. The cells (dots) are coloured according to the patient type. d: Feature plot displaying MYC expression on t-SNE of all cells in human heart. e: Violin plot showing the expression of MYC of all cells in human heart. f: t-SNE unsupervised clustering of fibroblasts in the human heart coloured by patient type. g: Gene Ontology analysis of differentially expressed genes. Genes were upregulated in human DCM CFs compared with those in human control CFs. h: Gene Ontology analysis of differentially expressed genes. Genes were downregulated in human DCM CFs compared with those in human control CFs. i: Feature plot visualising expression of MYC on t-SNE of fibroblasts. j: Violin plot showing the expression of MYC and CXCL1 in the six clusters.
