Fig. 7: Cellular communication between ATF3/ATF4⁺ ECs and LYVE1^hiMHCII^low macrophages is altered during PAD.

a,b, Heatmaps showing downregulated (a) and upregulated (b) ligand–receptor pairs in PAD. In each panel, the left part indicates the ligand log fold change (LFC) in ATF3/ATF4⁺ ECs and the right part the receptor LFC in LYVE1^hiMHCII^low macrophages from each ligand–receptor pair. Color indicates the LFC. c, Heatmap showing the regulatory potential of ATF3/ATF4⁺ ECs ligands upregulated in PAD (left labels) over the downstream target genes upregulated in LYVE1^hiMHCII^low macrophages (top labels). Color indicates the regulatory potential. d, Dot plot showing ORA results from the predicted targets in c. Color and size of the dots indicate −log₁₀(adjusted P value) and number of genes in each category (intersection size), respectively. e, Graphs showing the geomean expression ± s.e.m. of the markers CD206, CD80 and HLA-DR on human CD14⁺ cells cultured in vitro with increasing concentrations of EC-derived conditioned media (CM)—non-ischemic CM (gray, n = 3) and PAD CM (blue, n = 5). Fisher’s one-tailed test (as implemented in g:Profiler) was used in d. Two-way ANOVA (*P < 0.05, ***P < 0.001) was used in e. P = 0.0002 (e, CD206), P = 0.0001 (e, CD80), P = 0.0414 (e, HLA-DR). Min, minimum.

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