Extended Data Fig. 2: Expression of RPL3 and RPL3L in explanted patient hearts.
From: Pathogenetic mechanisms of muscle-specific ribosomes in dilated cardiomyopathy
a, RPL3 expression (mRNA-seq) normalized to average 60S genes. N = 2 technical replicates for D308N/T189M, 2 biological replicates for controls. Error bars signify s.d centered on mean value. Unpaired two-sided t-test. b, same as a but for RPL3L. c, RNA-seq read coverage (BAM file visualized in IGV genome browser) showing that both D308N and T189M variants are equally expressed. d, Intensity of the peptide containing the D308 residue in the T189M variant and the peptide containing the N308 residue in the D308N variant normalized to the total intensity in RPL3L. The T189 site was not detected as it lacks nearby trypsin sites. Mean value of N = 2 technical replicates ± s.d. Unpaired two-sided t-test. ns, not significant.
