Fig. 5: AZD1656 treatment reduces T cell-mediated cardiac inflammation and fibrosis in dbCM.

a, Circulating XXL cytokine plasma panel heatmap summary (control n = 10, db/db n = 12, AZD n = 10). Multiple group comparison by one-way ANOVA. b, Representative FACS contour plots. c, Relative frequency of myocardial CD4⁺ T cells quantified by FACS (control n = 12, db/db n = 12, AZD n = 7). Multiple group comparison by one-way ANOVA (control versus db/db P = 0.0001, control versus AZD P = 0.163, db/db versus AZD P = 0.002). d, Masson trichrome stained representative cardiac cross sections. e, Quantification of Masson trichrome fibrosis staining intensity (control n = 5, db/db n = 4, AZD n = 4). Multiple group comparison by one-way ANOVA (control versus db/db P = 0.0001, control versus AZD P = 0.128, db/db versus AZD P = 0.0002). f, Relative frequency of myocardial T_reg cells (CD4⁺CD25⁺FoxP3⁺) (n = 9 per group). Data are normalized to control (set to 1), and expressed as fold change. n, biological replicates (individual animals). Multiple group comparison by one-way ANOVA (control versus db/db P = 0.957, control versus AZD P = 0.02, db/db vereesus AZD P = 0.03). g, B cells, dendritic cells, neutrophils and macrophages (controls n = 8, db/db n = 8, AZD n = 5). Multiple group comparison by one-way ANOVA (B cells: control versus db/db P = 0.83, control versus AZD = 0.0082, db/db versus AZD P = 0.03; dendritic cells: control versus db/db P = 0.23, control versus AZD P = 0.70, db/db versus AZD P = 0.87; neutrophils: control versus db/db P = 0.89, control versus AZD P = 0.99, db/db versus AZD P = 0.99; macrophages: control versus db/db P = 0.77, control versus AZD P = 0.77, db/db versus AZD P = 0.44). *P < 0.05, **P < 0.01, ***P < 0.001. Multiple group comparison by ANOVA. n represents biological replicates (individual animals). Data are presented as mean ± s.e.m.

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