Extended Data Fig. 6: Dusp1 knockdown abolishes the ability of corticosterone to repress the proliferative potential of BMP7, FGF1 and OSM.
Analysis of cell proliferation by BrdU assay in neonatal (postnatal day 1 - P1) cardiomyocytes cultured in vitro following knock-down of Dusp1 for 48 h with/without subsequent stimulation with BMP7, FGF1 or OSM (10 ng/ml), and/or CORT (10−8 M). A pool of non-targeting scramble siRNAs (siSCR) was used as a negative transfection control. Cardiomyocytes were identified by cTnI staining and analysed by immunofluorescence for DNA synthesis (BrdU incorporation assay). Details on the number of replicates and experiments for each panel are provided in Supplementary Table 2. The values are presented as mean (error bars show standard deviation), statistical significance was determined using one-way ANOVA followed by Sidak’s test (comparison between pairs of treatments).
