Extended Data Fig. 7: Glucocorticoid receptor antagonism does not increase the mitogenic activity of NRG1 in postnatal day 1 cardiomyocytes.
Analysis of cell proliferation by BrdU assay in neonatal (postnatal day 1 - P1) cardiomyocytes cultured in vitro following stimulation with GR antagonist RU486 (10−7 M), alone or in combination with NRG1 (100 ng/ml). Cardiomyocytes were identified by cTnI staining and analysed by immunofluorescence for DNA synthesis (BrdU incorporation assay). Representative pictures are provided; arrows point at proliferating cardiomyocyte; scale bars 50 mm. Details on the number of replicates and experiments for each panel are provided in Supplementary Table 2. The values are presented as mean (error bars show standard deviation), statistical significance was determined using one-way ANOVA followed by Sidak’s test (comparison between pairs of treatments).
