Extended Data Fig. 9: Echocardiographic evaluation of additional morphological parameters of the in vivo mouse model of anthracycline-induced cardiotoxicity.

Analysis of (a) left ventricular anterior wall thickness at systole (LVAWs), (b) left ventricular end-systolic volume (LVESV), and (c) left ventricular end-diastolic volume (LVEDV) in adult mice at baseline and treated with doxorubicin (DOXO) alone or co-treated with NRG1, RU486, or NRG1 + RU486, 21 days after first DOXO injection. Details on the number of replicates and experiments for each panel are provided in Supplementary Table 2. The values are presented as mean (error bars show standard deviation), statistical significance was determined using one-way ANOVA followed by Sidak’s test in all panels (comparison between pairs of treatments).