Fig. 1: Overview of Fibrin-Based Nanoparticles (FBNs) and FBN Gels.

A Schematic comparing. material properties of high-density fibrin glues with those of FBN gels. Fibrin fibers are represented by purple ribbons, and FBNs are represented by small purple dots. The top panel depicts the high-density structure of fibrin glue which inhibits cell infiltration. The bottom panel depicts the high porosity of FBN gels, which allows for high cellular infiltration, as demonstrated by different cell types infiltrating the material. B Representative cryoSEM images of high-density fibrin glue, FBN gel, and a fibrin clot of physiologic density. Scale bar = 10 μm. C Representative confocal images of HUVEC cells seeded in high-density fibrin glue, FBN gel, and a fibrin clot of physiologic density after one week of growth, demonstrating relative cellular-level porosity. Scale bar = 200 μm. D Schematic of fibrin-based nanoparticle production. E Representative atomic force microscopy image of fibrin-based nanoparticle. Scale bar = 1 μm. F Representative image of lyophilized FBNs. Scale bar = 1 cm. G Representative images before and after rapid resuspension of lyophilized FBNs in deionized water via 10 seconds of mixing via vortex.