Fig. 2: Fibrin-Based Nanoparticle Gel Polymerization and Mechanical Properties in Response to Particle Concentration and Long-term Storage. | Communications Engineering

Fig. 2: Fibrin-Based Nanoparticle Gel Polymerization and Mechanical Properties in Response to Particle Concentration and Long-term Storage.

From: Colloidal-fibrillar composite gels demonstrate structural reinforcement, secondary fibrillar alignment, and improved vascular healing outcomes

Fig. 2: Fibrin-Based Nanoparticle Gel Polymerization and Mechanical Properties in Response to Particle Concentration and Long-term Storage.

Comparisons across all tests are between whole blood gels, high-density fibrin glue (HDFG) and whole blood-FBN gels of varying FBN concentration. A Average polymerization curves determined via rheometry for whole blood clots, high-density fibrin glue, and whole blood-FBN gels using differing concentrations of FBNs. Thrombin (0.5 U/mL) and CaCl2 (0.05 mM) and FBNs (varying final concentrations) were added to whole blood. N = 3. B Comparison of gelation time between whole blood clots, high-density fibrin glue, and whole blood-FBN gels with differing concentrations of FBNs. (C) Comparison of storage modulus after 15 minutes of polymerization between whole blood clots, high-density fibrin glue, and whole blood-FBN gels with differing concentrations of FBNs. (D) Schematic demonstrating Instron UTS methods utilized to measure tension, shear adhesion, and wound closure. E Comparison of tensile strength between gels. HDFG: N = 3, Other groups: N = 11-15 (F) Comparison of shear adhesion between gels. N = 7-11 G Comparison of wound closure rupture extension between gels. N = 8-10 (H) Schematic of material leak testing process. N = 7-15 (I) Comparison of leak pressure between biomaterials. J Comparison of degradation over four hours between gels. HDFG: N = 3. Other groups: N = 5. K Comparison of tensile strength of whole blood-FBN gel (330 M FBN/mL) made using newly polymerized FBNs or 1-year-old FBNs (lyophilized; room temperature (RT) storage). N = 11. L Comparison of adhesive strength of whole blood-FBN gel (330 M FBN/mL) made using newly polymerized FBNs or 1-year-old FBNs (lyophilized; RT storage). N = 9–13. M Comparison of leak pressure of whole blood-FBN gel (330 M FBN/mL) made using newly polymerized FBNs or 1-year-old FBNs (lyophilized; RT storage). N = 7. All comparisons were performed using one-way ANOVA with Tukey’s post hoc analysis or unpaired Student’s t-test. ns= p > 0.05, *=p < 0.05, **=p < 0.01, ***=p < 0.001.

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