Fig. 5: In vivo sensor measurements.
a Experimental setup for cold atmospheric plasma (CAP) treated murine wounds. b, d Current response curves produced for H2O2 and voltage response curves produced for oxidation-reduction potential (ORP) measurements, respectively. Sensor readings were obtained from wire sensors within the wounds of mice prior to (0 s) and directly after CAP treatment for the established suboptimal (60 s), optimal (180 s), and excessive (300 s) doses. Sampling time for each response curve is indicated by the gray region centered at 20 s. c, e H2O2 concentrations and ORP values derived from the current and voltage response curves, respectively, are displayed for each treatment group. Both figures show data points (black, diamond) for each group, with the statistical mean (clear, squares), median (black, line in the center of box plot), interquartile ranges (box range: 25–75th percentile of data set), and outer legs of each box (lower 5th percentile and upper 95th percentile of data set) being showcased. Outliers are shown above or below the outer legs (black, diamond). 27 data points are shown in c for 0 s, n = 9 for all treatment times. 12 data points are shown in e for 0 s, n = 3 for 60 and 180 s, n = 6 for 300 s. Statistical differences between 0 s and treatment groups are identified by: *p < 0.05; ****p < 0.0001. Exact p values can be found in supplemental data 1. One-way ANOVA with Tukey’s post-hoc test was used.
