Fig. 1: Diagram of intravital and fixed tissue imaging with fluorescence and SCoRe.
From: Label-free and fluorescence imaging of oligodendrocytes and myelin
a Schematic of the imaging hardware. A confocal microscope can be used to image both label-free SCoRe and fluorescent proteins or dyes up to ~200 µm below the pial surface. A multiphoton microscope can be used to image fluorescent proteins or dyes up to ~500 µm below the pial surface. b SCoRe, fluorescent proteins, and small molecule dyes can be used to image OPCs, oligodendrocytes and myelin in vivo. c SCoRe, fluorescent proteins, and small molecule dyes can also be combined with immunohistochemical staining in fixed tissue. d Label-free and fluorescent imaging of oligodendrocyte lineage cells and myelin can be used to probe mechanisms governing OPC proliferation and differentiation into myelinating oligodendrocytes, as well as myelin sheath plasticity.
