Fig. 2: Imaging oligodendrocyte lineage cells and myelin with genetically encoded fluorescent proteins.
From: Label-free and fluorescence imaging of oligodendrocytes and myelin
a Conditional expression of a membrane-anchored fluorescent protein in an OPC (left) and an oligodendrocyte (right). White arrows indicate cell soma. b Oligodendrocytes labeled with both a conditionally expressed cytoplasmic fluorescent protein and a constitutive membrane-tethered fluorescent protein. Arrows indicate an oligodendrocyte cell soma. c Longitudinal imaging of two OPCs differentiating. The top OPC successfully differentiates into a CNP+ oligodendrocyte while the bottom cell eventually dies. The yellow arrow indicates the newly differentiated Cnp-mEGFP+ oligodendrocyte and the white arrows indicate new myelin sheaths.
