Fig. 6: SCoRe imaging in fixed tissue.
From: Label-free and fluorescence imaging of oligodendrocytes and myelin
a Left: tiled image of a single z-plane from a fixed Cnp-mEGFP mouse coronal forebrain slice. Myelin can be visualized with both SCoRe and mEGFP fluorescence. Right: maximum projection of upper cortical layers, and single z-plane of the boxed area in (a). SCoRe signal overlaps with EGFP for myelin sheaths parallel to the imaging plane, but not for myelin sheaths orthogonal to the imaging plane. b Left: Tiled image of a single sagittal cerebellar z-plane from a fixed Cnp-mEGFP mouse. Myelin can be visualized with both SCoRe and mEGFP fluorescence. Right: maximum projection of the boxed area. SCoRe signal overlaps with EGFP in the granule cell layer (GCL) and white matter (WM), and both signals are absent from the molecular layer (ML), an unmyelinated area.
