Fig. 7: Combining SCoRe with genetically encoded fluorescent proteins and immunohistochemistry.
From: Label-free and fluorescence imaging of oligodendrocytes and myelin
a, b Combining SCoRe with Thy1-YFP and antibody labeling against Caspr allows the visualization of compact myelin and nodes of Ranvier along sparsely genetically labeled axons. c, d Breaks in SCoRe signal flanked by Caspr immunolabeling indicate a node of Ranvier. e SCoRe overlaps with antibody labeling against MBP. f SCoRe (white arrows) is excluded from the Ankyrin-G+ axon initial segment (AIS; red arrows). g SCoRe can be used to trace myelination patterns (t-SCoRe) along the axon (t-axon) of sparsely genetically labeled neurons.
